Marcos Vicente de Albuquerque Salles Navarro

• Abstract

  Recently, a nucleotide-based molecule, cyclic dimeric adenosine monophosphate (c-di-AMP), has been identified in several pathogenic bacteria, rapidly gaining the status of a central signaling controlling several essential bacterial processes, such as ion transport and DNA damage surveillance. In Staphylococcus aureus, it was found that c-di-AMP is essential for the cell wall homeostasis a…

  Development of new antibiotics against super-resistant Staphylococcus aureus strains: identification and characterization of inhibitors of the enzyme Diadenylate cyclase, AP.R

• Abstract

  In recent years a novel, nucleotide-based small molecule, c-di-GMP, has emerged in the spotlight of scientific investigation as a messenger unique to the bacterial world. The discovery that its intracellular levels strictly regulate cell adhesion and biofilm formation has related this molecule to a variety of disease states, including both chronic and acute bacterial infections. Protein d…

  Structural and functional studies of proteins involved in c-di-GMP-mediated signalling pathways , AP.JP

• Abstract

  Several bacterial species are able to form biofilms, which are cell aggregates attached to biotic or abiotic superficies. These biofilms are composed by cells shrouded by a matrix that hinders the diffusion of antibiotics and antibodies, and are therefore important for the resistance of pathogens. Furthermore, some cells can detach the biofilm under suitable conditions and start new sessi…

  Structural and functional characterization of the interaction between FleQ and FleN, transcription factors involved in the regulation of flagella biosynthesis in Pseudomonas aeruginosa, BP.PD

• Abstract

  Driven by environmental cues, bacterial transition from the planktonic state to the sessile state, in which biofilms are formed, is mediated by a control system within the bacterial cell that is not quite understood yet. It is known that switching between these phenotypes is closely related to intracellular levels of a small signaling molecule (c-di-GMP) - when these levels are high, they…

  Construction of a global map of protein-protein interactions in c-di-GMP signalling pathways of Pseudomonas aeruginosa and Xanthomonas axonopodis, BP.MS

• Abstract

  The pathways of biosynthesis and degradation of c-di-GMP are widely used by bacteria to coordinate the cellular machinery in the assimilation of phenotypes according to the environmental conditions. Some of them, such as virulence, biofilm formation and cell cycle control often cause obstacles in everyday human. A key regulator of such biological phenomena is a ubiquitous second messenger…

  Structural, biochemical and phenotypic studies of CHASE receptors associated with bacterial domains GGDEF / EAL, BP.DD

(Only some records are available in English at this moment)

• Abstract

  Pseudomonas aeruginosa is an opportunistic human pathogen capable to adapt and survive on a wide range of different environments. This adaptation ability is frequently associated with the formation of a sessile macrocolony embedded in a self-produced matrix, called biofilm. In Pseudomonas aeruginosa PA14, the major component of extracellular biofilm matrix is the PEL polysaccharide, a pol…

  Heterologous production of transmembrane complexes of the pel operon from Pseudomonas aeruginosa for functional and structural studies, BE.EP.MS

• Abstract

  Biofilm formation by microorganisms causes persistent infections to be resistant to treatment with anti-bacterial agents. As biofilm infections significantly contribute to patient morbidity and substantial healthcare costs, novel strategies to treat these infections are urgently required. Nucleotide second messengers, c-di-GMP and c-di-AMP, are major regulators of biofilm formation and as…

  Fragment-based screening for inhibitors of bacterial enzymes diguanylate and diadenylate cyclases using X-ray crystallography, BE.EP.PD