Abstract
The aim of this proposal is to characterize the transcriptome of sperm from sexed by density gradient centrifugation and flow cytometry. The experimental groups for semen and embryos going to be: 1) fresh semen, 2) control group: conventional semen thawed, 3) group gradient: density gradient centrifugation 3) group cytometry: sexed semen by flow cytometry. To obtain data on gene expressio…