Abstract
We obtained monoclonal antibodies against human osteosarcoma cells and osteoblastic like cells by immunizing mice with MG-63. After several steps of tests we sub cloned three monoclonal antibodies: PSP 4-5, PSP 42-22 and PSP 85-9. These antibodies recognize antigens which size are 100, 20 and 16 kDa, respectively. We evaluated the antigen expression in cryostat sections of undecalcified n…