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PROCESS OF SEX SELECTION OF MAMMAL SPERMATOZOA AND METHOD TO CONTROL QUALITY OF FROZEN SEXED SEMEN DOSES

Document type:Patente
Inventor(s): Vera Fernanda Martins Hossepian de Alameda Lima; Mariney Flávia Pereira Di-Tanno Ramalho; Carlos Alberto Moreira Filho
Applicant: Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Deposit date: February 2004, 11
INPI register:
PCT/BR2004/000009 - INPI Search
IPC: C12Q1/68 C12N C12N5/071 G01N33/50 G01N15/04 C12N5/00
Abstract

The invention allows the production by centrifugation in density gradient, in companies specialized in the production and commercialization of frozen semen, of semen doses enriched with X or Y chromosome-bearing spermatozoa, not impairing the fertilization capacity of said spermatozoa. According to the present invention, samples of spermatozoa containing both X and Y spermatozoa can be separated to produce subpopulations enriched with X or Y spermatozoa, which are substantially pure regarding the desired spermatozoa and substantially free from the other type of spermatozoa.


Grantee:Vera Fernanda Martins Hossepian de Lima
Principal researcher:Vera Fernanda Martins Hossepian de Lima
Institution: Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Faculdade de Ciências Agrárias e Veterinárias (FCAV)
Support type: Research Grants - Support for Intellectual Property Rights and Licensing (PAPI/Nuplitec)