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Molecular aspects of the effect of transforming growth factor-beta 1 (TGF-β1) in the signaling pathways in vitro biomineralization.

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Tatiani Ayako Goto Donato
Total Authors: 1
Document type: Doctoral Thesis
Press: São Paulo.
Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
Defense date:
Examining board members:
Victor Elias Arana Chavez; Alison Colquhoun; Patricia Gama; Arnaldo Rodrigues dos Santos Junior; Mario Sergio Soares
Advisor: Victor Elias Arana Chavez

This in vitro study aimed to evaluate the molecular effects of TGF-β1, with different supplementation time periods on the establishment of MC3T3-E1 cells, comparing with cells treated with AA+β-GP supplemented with Dex and/or TGF-β1, without or with neutralization of TGF-β1 receptors. The gene expression of the TGF-β1 and Smad3 were analyzed, as well as the osteoblast differentiation and biomineralization. The cells treated with TGF-β1 without neutralization of receptors have had inhibitory effect on some important stages of osteoblast differentiation and biomineralization in vitro, but expressed some important mineralization markers. Mineral nodules were observed in all treatments of cells with their TGF-β1 receptors neutralized, but there was a decrease in the expression of some important genes. The results confirm the complexity of the pathway signaling of TGF-β1, showing that there are gaps for understand the mechanisms of this molecule in the biology of osteoblasts. (AU)

FAPESP's process: 10/00352-6 - Molecular aspects of the effect of transforming growth factor - beta 1(TGF-b1)and glucocorticoids in vitro signaling pathways biomineralization
Grantee:Tatiani Ayako Goto Donato
Support type: Scholarships in Brazil - Doctorate