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Genetic variability and estimation of outcrossing rate of physic nut (Jatropha curcas L.) using molecular markers

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Eduardo de Andrade Bressan
Total Authors: 1
Document type: Doctoral Thesis
Press: Piracicaba.
Institution: Universidade de São Paulo (USP). Centro de Energia Nuclear na Agricultura (CENA/STB)
Defense date:
Examining board members:
Antonio Vargas de Oliveira Figueira; Carlos Augusto Colombo; Lee Tseng Sheng Gerald; Tsai Siu Mui; Giancarlo Conde Xavier Oliveira
Advisor: Antonio Vargas de Oliveira Figueira

Physic nut (Jatropha curcas L.) is a tropical tree that has acquired economic importance for the content of oil in its seeds and the possibility of its use for biofuel production. However, oil seeds and physic nut are toxic because of the presence of secondary metabolites such as phorbol esters which makes its use directly for human consumption and also its waste for animal feed difficult. The lack of commercial varieties and problems with pests and diseases are making physic nut cultivation in the world unattractive. The use of molecular markers such as ITS, PCR-RFLP, microsatellites and TRAP can help in studies of genetic diversity, aimed at developing varieties adapted to farmers needs. The aim of this study was to characterize the genetic variability of physic nut accessions deposited in the Germplasm Bank of the \'Universidade Federal de São Carlos\', in addition to allowing studies on the relationship among accessions, centers of diversity and reproductive system. Results are discussed highlighting that most diversity is concentrated among populations. The four markers used indicated that the center of diversity of this species is probably in America with emphasis on Mexico, Brazil and Colombia. The microsatellite markers indicate that physic nut has a mixed system of reproduction, combining self-pollinations, apomixis and crossing between related individuals which may explain the small genetic diversity found within populations. Due to the mixed system of reproduction and correlated mating, the collection of open-pollinated seeds for plant breeding or conservation should be conducted in a number of trees above 100 in order to ensure a structured sample (AU)

FAPESP's process: 07/04840-2 - Genetic variability and estimation of the outcrossing rate of the physic nut (Jatropha curcas L.) using molecular markers
Grantee:Eduardo de Andrade Bressan
Support Opportunities: Scholarships in Brazil - Doctorate