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Polyphasic characterization of xenobiotic-degrading bacteria diversity in Santos estuary

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Débora Frigi Rodrigues
Total Authors: 1
Document type: Master's Dissertation
Press: São Paulo. , ilustrações.
Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
Defense date:
Examining board members:
Vivian Helena Pellizari; Gabriel Padilla Maldonado; Maria Inês Zanoli Sato
Advisor: Vivian Helena Pellizari
Field of knowledge: Biological Sciences - Microbiology
Indexed in: Banco de Dados Bibliográficos da USP-DEDALUS; Biblioteca Digital de Teses e Dissertações - USP
Location: Universidade de São Paulo. Biblioteca do Instituto de Ciências Biomédicas; ICB T-ICB BMM QW4; R696c

Recalcitrant compounds such as polychlorinated biphenyls (PCBs) and hydrocarbons have attracted researcher’s attention all over the world, because of their persistence and side effects to the environment and to the humanity. The knowledge of genotypic an phenotypic biodiversity of xenobiotic compound degrading-microorganisms is extremely important for the comprehension of ecological aspects involved in microbial biodegradation; including the biodegrading-bacteria geographic dispersion that may also consider and provide a model for the comprehension of the patterns of geographic distribution of autochthonous microorganisms in the environment. Because of the presence of these compounds in Santos Estuary, it is very important the present study of microbial diversity in this environment. The purpose of this work is to investigate the occurrence and the bacterial diversity that are able to degrade PCBs and hydrocarbons in samples of the Santos Estuary. For that, samples were collected and analyzed using traditional techniques of culturing and chemical analyses of PCBs and hydrocarbons biodegradation. Molecular Biology techniques were also used to determine the presence of catabolic genes, to study the different genotypic patterns of BOX-PCR and to identify the microorganisms. As a result, 61 isolates were obtained, and then they were amplified with the BOX-PCR primer resulting in 30 amplicons with 28 different band patterns. These isolates were then tested for 6 distinct catabolic genes with positive results for the todC1 (toluene), ndoB (naphthalene), xylE (xylene) and bphA (biphenyl) genes. In the degradation tests, 12 PCBs degrading-isolates and 44 hydrocarbons degrading-isolates were obtained. As a result of the identification, was obtained 11 different genera of bacteria. Besides, most of the microorganisms belonged to the Gamma Proteobacteria subdivision. As a conclusion of these results and from the chemical and physical parameters studied in this work, was possible to notice that the studied area presents a noticeable potential for bioremediation with hydrocarbons and PCBs autochthonous degrading-microorganisms. (AU)

FAPESP's process: 00/03470-8 - Polyphasic characterization of the biodiversity of degrading isolates of xenobiotic pollutants in the Baixada Santista
Grantee:Debora Frigi Rodrigues
Support Opportunities: Scholarships in Brazil - Master