Advanced search
Start date

Expression and production of ECP by atypical enteropathogenic Escherichia coli.

Full text
Danielle Dias Munhoz
Total Authors: 1
Document type: Master's Dissertation
Press: São Paulo.
Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
Defense date:
Examining board members:
Roxane Maria Fontes Piazza; Tania Aparecida Tardelli Gomes do Amaral; Marcia Pinto Alves Mayer
Advisor: Roxane Maria Fontes Piazza

Atypical enteropathogenic E. coli (aEPEC) is an pathotype that causes diarrhea. aEPEC does not produce BFP, suggesting that other pili must be involved in aEPEC adhesion to the host cell. ECP is found in most pathogenic and non-pathogenic E. coli. The objective of this study was to evaluate the expression and production of ECP in aEPEC. The presence of ecp operon was assessed by PCR and the expression evaluated by PCR using cDNA obtained from bacterial growth in LB, DMEM and preconditioned DMEM. ECP production was evaluated by immunofluorescence. It was observed that the operon expression by all strains only occurred when they were grown in preconditioned DMEM. There was differential expression of ecp operon when strains were grown in LB or DMEM. ECP production was observed only by one strain when grown in LB. Three strains producted ECP grown in DMEM, but there was a higher production of the pili when strains were grown in DMEM preconditionated. These results suggest that cellular signaling may interfere with the expression and production of ECP. (AU)

FAPESP's process: 13/19784-1 - Characterization of the localized adherence-like pattern in atypical enteropathogenic Escherichia coli displaying different genetic fimbrial profiles.
Grantee:Danielle Dias Munhoz
Support Opportunities: Scholarships in Brazil - Master