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Functional and Structural analysis about sulfate ABC transporter in Xanthomonas citri

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Cristiane Tambascia Pereira
Total Authors: 1
Document type: Doctoral Thesis
Press: Campinas, SP.
Institution: Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia
Defense date:
Examining board members:
Andrea Balan; Alessandra Alves de Souza; Jörg Kobarg; Adriana Santos Soprano; Rita de Cássia Café Ferreira
Advisor: Andrea Balan

Xanthomonas citri sp. citri is a phytopathogenic bacteria causes citrus canker in citrus. This disease causes important economic losses for Brazil because this country is on of most exporter for orange juice and no have effective control disease. In. X. citri has lack information about ion uptake in general, include sulfate, essential element for grown in all types of life. Previous studies described the high affinity sulfate ABC transporter and this assimilation pathway is complete in X. citri and activated during grown in vitro and in vivo. To continue this studies, this work was proposed to characterize, functional, and structural insights for ABC Transporter SbpCysUWA. The periplasmic compound was solved at 1.14 Å with sulfate into the pocket .allowing to understand the ligation coordination. On the other hand, functional studies with knockout for Cys U permease reduces gum xanthan production and xanthomonadin but not the ability to grown culture in vitro. In addition, leaf infections of five different citrus varieties presented by a deletion, affect the infection period but not the cancer phenotype. These data other high affinity ABC transporters, such as the Ssu (1) and Ssu (2) alkanesulfonates, characterized by the group in the Sulphur starvation in this period. Moreover, were identified three putative sulfate carrier with low ion affinity. RT-PCR data showed this genes is transcripts in LB ON (expects sac1 gene) and inducing media XAM1( in vitro). These data show that the bacteria uses all its resources to maintain sulfur levels during its growth. Finally, this work has opened a series of perspectives for future studies on these transporters, both from a functional and structural insights (AU)

FAPESP's process: 13/16094-4 - Functional and structural studies about the sulfate ABC transporter in Xanthomonas citri
Grantee:Cristiane Tambascia Pereira
Support Opportunities: Scholarships in Brazil - Doctorate