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Evaluation of the tissue viability of fresh periodontal ligament under the influence of enamel matrix proteins and their angiogenic potential

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Carla de Oliveira Pires da Silva
Total Authors: 1
Document type: Master's Dissertation
Press: Ribeirão Preto.
Institution: Universidade de São Paulo (USP). Faculdade de Odontologia de Ribeirão Preto (PCARP/BC)
Defense date:
Examining board members:
Mario Taba Junior; Raquel Mantuaneli Scarel Caminaga; Moira Pedroso Leão; Paulo Tambasco de Oliveira
Advisor: Mario Taba Junior

Periodontitis is a chronic inflammatory disease, multifactorial and highly prevalent in the world population that affects the teeth of the supporting tissues, generating sequels difficult to solve even with the most modern regenerative techniques. Tissue therapy appears to be a promising alternative and undifferentiated cells of the periodontal ligament (PDL) have shown great therapeutic potential. However, the PDL needs to appropriate stimuli biomodificatores that differentiation occurs in a coordinated fashion. The enamel matrix proteins (EMP) are a type of biomodificator that promotes new cementum formation. Despite being used clinically, its association with PDL fresh cells has not yet been explored. This is a study of gene and protein expression of PDL with reuse purpose of the newly extracted tissue stimulated by EMD, with a view to periodontal regeneration. The results show VEGF gene expression (p = 0.5194) difference in median -001 and FGF-2 (p = 0.0059) difference in medians of -0.4167. In the study of cytokines, VEGF-A (p <0.0001) with the difference between medians of 60.93, whereas VEGF-D (p = 0.0049) with from 2.45 medians. Through the results of the FGF-2-based gene expression and protein-based VEGF-A, it was observed that the EMD modulated the tissue response ex vivo in the 10-minute period considertingthe standard angiogenic. This combination may serve as a therapeutic approach aimed at future clinical application of tissues commonly discarded after extraction, such as the PDL. (AU)

FAPESP's process: 13/25990-3 - Analysis of fresh cells of the periodontal ligament and the biomodificador effect aiming the regeneration of the periodontal tissue
Grantee:Carla de Oliveira Pires da Silva
Support Opportunities: Scholarships in Brazil - Master