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Histological changes of intestinal epithelium of juveniles dourado Salminus brasiliensis fed diet with vegetables proteins sources alternatives

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Author(s):
Thaline Maira Pachelli da Cruz
Total Authors: 1
Document type: Doctoral Thesis
Press: Piracicaba.
Institution: Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC)
Defense date:
Examining board members:
José Eurico Possebon Cyrino; Margarida Maria Barros; Urbano dos Santos Ruiz
Advisor: José Eurico Possebon Cyrino
Abstract

Economic and environment constraints have brought the need to replace fishmeal (FM) in diets of aquatic organisms by less expensive feedstuff, especially dietary protein sources. However, such protein sources generally of plant origin, e.g. soybean meal, have antinutritional factors that can negatively affect digestive system of the fish, impairing health and consequently, production. To identify possible effects of plant protein sources in the diets for carnivore fish, this study evaluated effects of various dietary plant protein sources on digestibility, performance, histology of the intestinal epithelium of the carnivore, Neotropical Characin dourado, Salminus brasiliensis, fed plant protein-based diets. A digestibility trial was carried out in standard protocol using diets with inert marker chromium oxide III (Cr2O3) and modified system Guelph for feces. A performance test evaluated the use of three plant protein sources, soybean meal (SBM), cottonseed meal (CTM) and peanut meal (PTM) as surrogate protein source to fishmeal in a practical diet in five levels inclusion: 0 (control); 25.0%; 50.0%; 75.0% and 100%, in a randomized block design (n=3). Samples of tissue of intestinal tract of fish were collected in the distal segment at 20 and 40 days of the feeding period, and analyzed for histological and morphological characteristics. No differences were recorded for apparent digestibility coefficients (ADC) of feedstuff and energy and protein of feedstuffs. Dietary CM and PM elicited better feed consumption comparatively to the other feedstuff, Fish fed diet diet SBM50 presented reduction of intestinal folds at 20 days, but at 40 days had increased folds height, lamina propria thickening, and increased goblet cell density. Considering a scanning microscopy, evident signs of enteritis were registered. The alteration in the morphology and histology of the intestinal epithelium reflected the interaction of effects of the antinutritional factors present in each vegetable protein source, resulting in lower weight gain, growth and decreased intestinal homeostasis, even though best feed consumption and ADC were registered for fish fed CTM and FM.Concluded that SBM caused enteritis and CTM and PM can be used in feeding dourado. (AU)

FAPESP's process: 14/15618-2 - Intestinal ephitelium and microbiota of juveniles pacu Piaractus mesopotamicus and dourado Salminus brasiliensis fed diet with vegetable protein sources
Grantee:Thaline Maira Pachelli da Cruz
Support Opportunities: Scholarships in Brazil - Doctorate