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Effect of insulin-like growth factor I on the in vitro infection of mouse peritoneal macrophages by Leishmania L. amazonensis

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Author(s):
Anderson Guilherme dos Santos Barssotti
Total Authors: 1
Document type: Master's Dissertation
Press: São Paulo.
Institution: Universidade de São Paulo (USP). Instituto de Medicina Tropical de São Paulo (IMT)
Defense date:
Examining board members:
Hiro Goto; Roberto Mitsuyoshi Hiramoto
Advisor: Hiro Goto
Abstract

In Leishmania infection the immune response begins soon after the inoculation of promastigotes in the individual. In this context will be the participation of several factors of the innate immune response that will direct to an adaptive immune response responsible for the evolution of the disease. One of these factors that participates in this parasite-host interaction is the insulin-like growth factor I (IGF-I). It has been shown that extrinsic IGF-I favors parasite proliferation and infection progression. However, IGF-I is constitutively present in macrophages. In this work we evaluated the expression of IGF-I, parasitism and nitric oxide production in murine macrophages infected with Leishmania (L.) amazonensis and the effect of IGF-I inhibition on parasitism after IGF-I silencing by RNA from interference. Peritoneal macrophages were infected for 2 and 4 hours with L. (L.) amazonensis promastigotes in the presence of 5% fetal bovine serum (FBS) and 0.5% Bovine Serum Albumin (BSA) in the presence or absence of IGF-I small-interfering RNA (siRNA) and liposome (Lipo). Cells were washed and then maintained in culture medium for 24, 48 and 72 h. When the recombinant IGF-I was added separately during the initial incubation the parasitism increased relative to the control. When the siRNA was added there was a decrease in IGF-I expression and consequently a decrease in parasitism in relation to the control. The results obtained suggest an important role of IGF-I in the infection of murine mouse peritoneal macrophages by Leishmania (L.) amazonensis. (AU)

FAPESP's process: 13/03668-2 - Effect of insulin like growth factor I in the infection of mice peritoneal macrophages in vitro by Leishmania (L.) amazonensis
Grantee:Anderson Guilherme dos Santos Barssotti
Support Opportunities: Scholarships in Brazil - Master