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Osteogenesis in vitro from human dental pulp stem cells: role of matrix metalloproteinases and their inhibitors.

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Author(s):
Letícia Miquelitto Gasparoni
Total Authors: 1
Document type: Master's Dissertation
Press: São Paulo.
Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
Defense date:
Examining board members:
Katiucia Batista da Silva Paiva; Cecilia Helena de Azevedo Gouveia Ferreira; Maria Fernanda Pereira de Araújo Demonte Forni
Advisor: Katiucia Batista da Silva Paiva
Abstract

Bone loss is a major public health problem throughout the world and are not ideal bone substitute. Bone bioengineering comes as a new therapeutic approach and is based on cells, biomaterials and signaling molecules. Mesenchymal stem cells have become very attractive due to their osteogenic potential. Thus, knowledge of the profile of secreted molecules and mechanisms that control both undifferentiated state and during osteogenesis is required. Thus, our objective was to evaluate the expression profile of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs and RECK) and its function during induction of osteogenesis in vitro from human dental pulp stem cells (DPSCs). Some MMPs, especially MMP-2 and MT-MMPs and their inhibitors, are expressed in undifferentiated DPSCs. During osteogenesis, the levels of transcripts were positively modulated in relation undifferentiated DPSCs and protein levels of MMP -2 and -14 are higher and related to mineralization phase. Therefore, we suggest that MMPs/TIMPs/RECK may play role in the maintenance of the undifferentiated state of DPSCs and may be important for osteogenesis and mineralization in vitro. (AU)

FAPESP's process: 14/01395-1 - Osteogenesis in vitro and in vivo from human dental pulp stem cells: role of matrix metalloproteinases and their inhibitors
Grantee:Letícia Miquelitto Gasparoni
Support type: Scholarships in Brazil - Master