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Molecular study of the development of Puccinia psidii Winter in vitro and during its infection in Eucalyptus grandis

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Andressa Peres Bini
Total Authors: 1
Document type: Doctoral Thesis
Press: Piracicaba.
Institution: Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC)
Defense date:
Examining board members:
Carlos Alberto Labate; Luis Eduardo Aranha Camargo; Esteban Roberto González; Diego Mauricio Riaño Pachón
Advisor: Carlos Alberto Labate

Brazilian production of Eucalyptus spp. is one of the greatest in the world but it has been affected by Puccinia psidii Winter, the causal agent of eucalyptus rust. The comprehension of molecular mechanisms of pathogenicity and chemical composition of resistant and susceptible eucalyptus plants as well as having a molecular tool for early detection of the disease in field can be used for the development of improved control strategies against this phytopathogen. Molecular studies of P. psidii is limited because it is an obligate biotrophic fungus with limited in vitro development. Biotrophic fungi investigations are made mainly in planta at late developmental stages of the disease. This way, most information of early stages of infection as the development of specialized structures of biotrophic fungi are little understood. The study of early stages of the infection process of biotrophic fungi in planta is hampered by the high amount of plant material in relation to fungi material which is difficult to be obtained in an isolated form for analysis. In this work we developed a protocol to increase in vitro germination and structural differentiation of P. psidii and used this protocol to obtain isolated fungi material for the identification of candidate genes related with virulence factors and initial structural morphogenesis. Moreover, we analyzed the composition of metabolites present in the cuticular wax of leaves from resistant and susceptible E. grandis plants and developed a methodology for the detection of P. psidii in asymptomatic leaves of Eucalyptus grandis. Data obtained in this work help the comprehension of E. grandis-P.psidii interaction at early stages of the infection process and can be used for the development of improved control strategies of eucalyptus rust. (AU)

FAPESP's process: 13/07596-6 - Molecular study of the pathogenic fungus Puccinia psidii Winter, the causal agent of rust, in vitro and during their interaction with Eucalyptus spp
Grantee:Andressa Peres Bini
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)