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AHNAK regulates the formation and exchange of extracellular vesicles from breast tumor cells and fibroblasts.

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Author(s):
Thaiomara Alves Silva
Total Authors: 1
Document type: Doctoral Thesis
Press: São Paulo.
Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
Defense date:
Examining board members:
Vanessa Morais Freitas; Carolina Beltrame Del Debbio; Adriana Franco Paes Leme; Claudimara Ferini Pacicco Lotfi; Paulo Caleb Júnior de Lima Santos
Advisor: Vanessa Morais Freitas
Abstract

The successful development of tumors is not only dependent on cell mutations, but also driven by the tissue microenvironment; relies on interaction of cells and their surrounding stroma. Some cell types release vesicular structures into the extracellular space that would be involved in cellular communication and tumor progression. The aim of this study was to analyze vesicle-mediated interactions between tumor cells and normal fibroblasts. Tumor cells were plated above fibroblasts monolayer and both loaded with different vital dyes. Our results evidenciated presence and exchange of vesicles between breast tumor cells and fibroblasts in co-culture. Vesicles isolated showed heterogeneous sizes. Tumor cell showed more vesicles than normal cells. These vesicles were composed of AHNAK and Annexins proteins. The protein AHNAK was detected in exchanged vesicles and was increased in tumors when compared to normal breast tissues. AHNAK could represent a vesicle structural molecule that would influence breast tumor biology. (AU)

FAPESP's process: 11/09472-7 - AHNAK regulates formation and exchange of extracellular vesicles between breast tumor cells and fibroblasts
Grantee:Thaiomara Alves Silva
Support type: Scholarships in Brazil - Doctorate