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Influence of steroid hormones in the migration, invasion and expression of ADAMTS 1 and 4 proteases in ovary cancer cells.

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Author(s):
Maíra de Assis Lima
Total Authors: 1
Document type: Master's Dissertation
Press: São Paulo.
Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
Defense date:
Examining board members:
Vanessa Morais Freitas; Maria Ines Borella; Alexandre Bruni Cardoso
Advisor: Vanessa Morais Freitas
Abstract

Ovarian carcinoma is the leading cause of gynecological neoplastic death, being associated primarily with deregulation of sex hormones. ADAMTS are secreted proteases. Our aim is to assess whether sex hormones would affect ADAMTS1 and 4 expressions in ovarian cancer cells. Estrogen and testosterone induced a decrease on gene expression of ADAMTS 1 and 4 compared to the control for the ES-2 cell line, while progesterone led to an increase in the mRNA levels of these same proteases. Progesterone increases ADAMTS\'s protein levels in the lysate and in conditioned medium from NIH-OVCAR-3 and in the lysate from ES-2 cells. Immunofluorescence showed ADAMTS 1 located at the cell nucleus. NIH-OVCAR-3 cells treated with progesterone exhibited decrease migratory activity compared to control and ES-2 exhibited decrease invasion activity. We conclude that progesterone modulates ADAMTS1 and 4 levels in ovarian cancer cell lines and decrease migratory and invasion behavior in ovarian cancer cells. (AU)

FAPESP's process: 13/01092-6 - Influence of steroid hormones in expression levels and location of ADAMTS-1, 4 and 9 in cells derived from ovarian tumors
Grantee:Maíra de Assis Lima
Support type: Scholarships in Brazil - Master