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Biomarker transferability from parasite-rodent models into a human sub-Saharan population via targeted UPLC-MS metabolomic analysis

Grant number: 10/01579-4
Support type:Scholarships abroad - Research
Effective date (Start): March 15, 2010
Effective date (End): March 14, 2011
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
Principal researcher:Marina Franco Maggi Tavares
Grantee:Marina Franco Maggi Tavares
Host: Elaine Holmes
Home Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Research place: Imperial College London, England  

Abstract

In an initial approach to evaluate health issues in developing countries, the parasite-focused consortium comprised of the Biomolecular Medicine at the Imperial College London (www1.imperial.ac.uk/medicine/ about/divisions/surgeryandcancer/divisionofsurgery/biomol_med/) and the Swiss Tropical Institute (www.swisstph.ch/) enabled the establishment of seven different parasite-rodent models (Trypanosoma brucei brucei, Plasmodium berghei, Schistosoma mansoni, Schistosoma japonicum, Echinostoma caproni, Fasciola hepatica and Trichinella spiralis). By using NMR spectroscopy-based metabonomic approaches, a cross-comparison of the extracted biomarkers in all the models provided information about the specificity of biomarkers with respect to a particular infection, stability over time, diagnostic significance among others.The aim of this proposal is to evaluate the transferability of candidate biomarker previously identified from single-parasite rodent models into a human sub-Saharan population by performing metabolite targeted analysis via UPLC-high resolution MS-based methods. As a study model, urine samples from 100 well characterised school children from Côte d'Ivoire, Africa, bearing multiple diseases including P. falciparum-derived malaria, schistosomiasis and other helminth infections, drug treatments and other reported patho-physiological factors (e.g. anaemia, ikterus, hepato- and splenomegalie, etc.) were selected. Samples were collected at two different time points, i.e. children were pre and post treated for the most prevalent parasitic infections, whereby intermittent preventive treatment with sulfadoxine and pyrimethamine was applied against malaria and a combination chemotherapy of albendazole/praziquantel against soil-transmitted helminths and schistosomiasis, respectively. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SARIC, JASMINA; WANT, ELIZABETH J.; DUTHALER, URS; LEWIS, MATTHEW; KEISER, JENNIFER; SHOCKCOR, JOHN P.; ROSS, GORDON A.; NICHOLSON, JEREMY K.; HOLMES, ELAINE; TAVARES, MARINA F. M.. Systematic Evaluation of Extraction Methods for Multiplatform-Based Metabotyping: Application to the Fasciola hepatica Metabolome. Analytical Chemistry, v. 84, n. 16, p. 6963-6972, . (10/01579-4)

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