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Lead quantification and speciation in blasted rats using atomic spectrometry methods: correlation among metal concentration, speciation and physiological disturbances

Grant number: 09/15913-6
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): February 01, 2010
Effective date (End): March 31, 2011
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
Principal researcher:Solange Cadore
Grantee:Rafael Arromba de Sousa
Home Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated research grant:06/59083-9 - Inductively coupled plasma mass spectrometry: fundamentals and applications, AP.TEM


Sample preparation procedures for different tissues of rats, previously intoxicated with lead, will be studied. The total lead concentration in these tissues will be determined by GF AAS and the lead speciation will be carried out using the HPLC-ICP-MS technique. Wistar-Hannover rats will be employed as experimental animals that will receive successive lead acetate doses. After that, the presence of lead in the following tissues will be investigated: heart, liver, kidney and bones. For the different tissues appropriate sample treatments will be established for the two analysis type (total and lead speciation). Optimum instrument conditions will also be evaluated for both atomic absorption and mass spectrometers. The lead distribution among experimental animal tissues is not reported in the literature and this kind of information will be used to correlate the presence of lead with physiological effects that have being observed in rats. This study is a cooperative work between researches in analytical chemistry and medicine. The results of this project may contribute for the understanding of the lead intoxication mechanism and consequently to review the current procedures used for detoxification treatments, which is an important issue for public health.

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