Scholarship 06/00246-6 - Microbiologia industrial, Fermentação alcoólica - BV FAPESP
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Yeasts from alcoholic fermentation monitoring through killer toxins sensitivity profile

Grant number: 06/00246-6
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Start date until: May 01, 2006
End date until: April 30, 2008
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Sandra Regina Ceccato Antonini
Grantee:Ana Paula Guarnieri Bassi Alvarenga
Host Institution: Centro de Ciências Agrárias (CCA). Universidade Federal de São Carlos (UFSCAR). Araras , SP, Brazil

Abstract

During the alcoholic fermentation, the starter inoculum is continuosly recycled and subject to contamination from the environment. Microbiological monitoring is needed, specially the ones which discriminate the strains and are fast and inexpensive. The killer reaction was discovered 40 years ago in S. cerevisiae, and satisfactory results have already been obtained using the killer sensitivity for yeast characterization. Early studies pointed out favorably to the development of a methodology for biotyping to be used for yeast monitoring in alcohol industry, with the selection of nine killer yeasts to which industrial yeast strains presented characteristic sensitivity profile, depending on the group which belong (S. cerevisiae or non Saccharomyces). This work aims the optimization of production, purification and concentration of nine killer toxins, from strains isolated from the alcoholic fermentation, with the objective of a kit of biotyping for use in laboratories and industries.

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