The PANDER/FAM3B (pancreatic-derived factor) was recently identified as a novel cytokine expressed capable to induce apoptosis in insulin secreting beta-cells. However, PANDER gene is also expressed in prostate and breast tumors. Our goal is to evaluate the pro-apoptotic properties of PANDER in prostate carcinoma (DU145) and breast adenocarcinoma (MDA-MB-231) stably over-expressing PANDER by lentiviral-mediated infection. The ability of PANDER to induce apoptosis will be evaluated by DNA fragmentation analysis, changes in cell membrane, caspase activity and apoptosis-related gene expression. Our results can be contributing to better understand the functional role of this novel cytokine in the control of tumoral progression.
News published in Agência FAPESP Newsletter about the scholarship: