Advanced search
Start date
Betweenand

Importance of integrins as oocytes and/or sperm adhesion molecule during fertilization and their relationship with bull fertility

Grant number: 06/06008-0
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): March 01, 2007
Effective date (End): February 28, 2010
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal researcher:Valquiria Hyppolito Barnabé
Grantee:Roseli Fernandes Gonçalves
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The molecular events of sperm-oocyte binding and fusion have been studied extensively, but identification of the molecules involved in sperm-oocyte interaction remains incomplete. Integrins are a family of heterodimeric cell adhesion molecules that mediate cell-cell and cell-extracellular matrix interactions. Although integrins have associated with the plasma membrane of some mammal oocytes and spermatozoa, the presence of these molecules on the surface of bovine oocytes and spermatozoa has not been reported. The aim of the present study will be: a) identification of several bovine oocytes and spermatozoa integrins subunits; b) investigate whether integrins are required for bovine sperm-oocyte binding and fusion process; c) investigate the role of integrins in fertilization; d) study if the expression of some integrins on bovine spermatozoa will be altered in the infertile bulls during in vitro fertilization. It will be used the confocal microscopy to identify integrins subunits on the surface of bovine oocytes and spermatozoa; experiments to investigate the role of integrins during fertilization and sperm-oocyte binding; flow citometry to investigate the spermatic quality.

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

Please report errors in scientific publications list by writing to: cdi@fapesp.br.