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Characterization of proteins in some foods with potential for use in ruminant feed

Grant number: 09/09726-9
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): August 01, 2009
Effective date (End): July 31, 2010
Field of knowledge:Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
Principal Investigator:Antonio Fernando Bergamaschine
Grantee:Tamyres Rodrigues de Amorim
Host Institution: Faculdade de Engenharia (FEIS). Universidade Estadual Paulista (UNESP). Campus de Ilha Solteira. Ilha Solteira , SP, Brazil


The second most nutrients required by animals are the proteins, whose main sources are the co-product of oil production. The Brazilian industrial park food is modest in the generation of co-products of protein sources, while the demand is quite high, making the protein the nutrient of greatest relative cost of a ration. With the advent of the supply of biodiesel co-product sources protein increased. As proteins that can reach the intestines of ruminant animals are originated from microbial protein synthesized in the rumen from the rumen degradable protein (RDP) and rúmen um not protein (RUP). At least 50% of amino acids available for absorption in the intestine are originated from microbial protein that is high quality and relatively constant composition. The systems of adequacy of diets for ruminants require knowledge of the degradability and intestinal digestibility of protein foods, which are obtained by techniques "in situ" and "in vitro" of three stages, respectively. Will be evaluated some unusual foods as soybean silage, silage of high moisture corn, silage mixed sugar cane with soybean grain (50:50), of raw pigeon pea, raw cassava, residue of the extraction of palm pupunha: sheath (from bark) and leaves, legume and cotton cake, besides the co-products of oil extraction to biodiesel: pie of forage turnip and pinion gentle rise of two, and Crambe. The wet food will be pre-dried at 55-60 ° C for 72 hours and ground to pass on the 2 mm sieve, five grams of each food will be packaged in bags of nylon (Tenyl) with pores of 50 microns and with measures of 8x16 cm sealed with sealing machine. Five animals will be fitted with rumen cannulas and time of incubation will be 0,2,8,16,24 and 48 hours and concentrated and thins 72 hours for roughage. The effective degradability will be obtained as: In = a + (bc) / (c + k). The intestinal digestibility (ID) of RUP will be made "in vitro" in three stages as follows: a sample containing 15 mg of nitrogen will be incubated with HCL and pepsin, followed by addition of NaOH and subsequently in the KH2 PO4 buffer solution containing tymol and pancreatin. After incubation will be filtering on filter paper to obtain the undigested residue. The DI of the RUP (%) is calculated as: DI (%) = [(N incubated mg - mg of N in the residue): mg N incubation)] x 100 x 6.25.

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