Evaluation of plant active agents potential in wild and genetically modified insect cells cultivation

Abstract

The present work intends to study the potential of plant active agents, such as rosemary (Rosmarinus officinallis) and ginger (Zingiber officinale) active agents, which present already demonstrated antioxidant properties, in the culture of wild and genetically modified insect cells for the production of vaccine components. Embryonary cells of the dipteran Drosophila melanogaster Schneider 2 (S2) cell will be employed, which are used with success for the expression of a variety of biomolecules, such as the hepatitis B virus surface antigen (HBsAg), proteins of the human immunodeficient virus (HIV), dopamine, gamma amino butyric acid (GABA), opioid receptors and human plasminogen. The cells which will be employed will be specifically the wild and transfected lines used for the production of the G glycoprotein of the rabies virus and the hepatitis B virus surface antigen (HBsAg). This expression system is capable to perform post-translational modifications in the required final complexity, and these cells are easier to culture than mammalian cells, involving also less costs. The present study proposes the evaluation of the potential of rosemary and ginger active agents obtained through supercritical technology and their delivery through a dispersion in liposomes on the metabolism, growth, cellular productivity and in the production of the mentioned proteins. Amongst the important properties of the extracts in focus, it is expected to verify their effects in cellular behavior related to apoptosis, delaying the process and, consequently, increasing the global productivity of the system.