Study of the quantification of endothelial progenitor cells in animals with acute kidney injury practicing intense physical exercise

Abstract

Stem cells (SC) are capable of proliferation, self-renewal in different lineages and differentiation into mature cells and functional. Bone marrow is the main reservoir of the SC mesenchymal cells which differentiate into endothelial progenitor cells (EPCs) and can be mobilized into the bloodstream after a vascular injury. Exercise (EXE) is an important factor that acts in the recruitment of these cells, which in turn promote neovascularization by differentiating into endothelial cells. Therefore, the objective of our work is to study the effect of summation of intense physical exercise and administration of EPCs in a state of progression of renal disease and the development of acute renal failure (ARF) disease. Wistar rats will be divided into the following groups: 1) control, 2) Exe, 3) IRA, 4) IRA + Exe, 5) + IRA Injection of EPCs, 6) IRA + + Exe Injected EPCs. The IRA is surgically induced ischemia / reperfusion and physical activity will consist of high-intensity swimming held 5 times a week. To study the function will be taken 24-hour urine in metabolic cages and will be assayed: protein, urea, creatinine. The EPCs are isolated from bone marrow of rats, characterized by immunofluorescence using antibodies to factor VIII, CD34 and VEGFR2, to be later injected (ev) in the treated groups. At the end of the protocol, the kidneys will be sent to morphological analysis through stained with hematoxylin and eosin and Masson, and blood samples from the animals will be taken to measure the number of circulating EPCs by flow cytometry. Will be held renal immunohistochemistry with anti CD34 and VEGFR2 to analyze the presence of EPCs in this tissue.