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Osteoclastogenesis and matrix metalloproteinase-9 expression in apical and periapical repair following root canal treatment

Grant number: 09/16882-7
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): February 01, 2010
Effective date (End): December 31, 2011
Field of knowledge:Health Sciences - Dentistry - Pediatric Dentistry
Principal Investigator:Francisco Wanderley Garcia de Paula e Silva
Grantee:Renato Petille
Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


Matrix metalloproteinases (MMPs) are enzymes capable of tissue degradation during inflammatory events. These enzymes are expressed in human periapical cysts and granulomas in high concentration. However, MMP modulation following root canal treatment is not known. Therefore, the aim of this study will be to evaluate the expression of MMP-9 in periapical area in teeth with apical periodontitis and following root canal treatment using several clinical protocols. Then, the expression of MMP-9 will be correlated to periapical osteoclastogenesis. Apical periodontitis will be induced in dog’s teeth then the root canal treatment will be performed in a single visit or using calcium hydroxide as root canal dressing. Vital teeth submitted to root canal treatment, teeth with apical periodontitis not treated and healthy teeth will be used as controls. One hundred and eighty days following root canal filling, the animals will be euthanized and the tissues will be removed and submitted to histopathological evaluation. To investigate if tissue destruction is associated with MMP-9 expression, immunohistochemistry and western blotting will be performed. The presence and activity of osteoclasts will be determined by cell counting using tartarate-resistant acid phosphatase labeling (TRAP). Groups will be compared using one-way ANOVA or Kruskal-Wallis and by Fisher exact test (alpha = 0.05). (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LÉA ASSED BEZERRA DA SILVA; KLÉBER CORTÊS BONIFÁCIO; FERNANDA SOUZA LIÉVANA; GIOVANA GONÇALVES MARTINS; DANIEL SILVA-HERZOG FLORES; MÁRIO ROBERTO LEONARDO; RAQUEL ASSED BEZERRA DA SILVA; FRANCISCO WANDERLEY GARCIA PAULA-SILVA. Apical Periodontitis Healing Following Treatment is Impacted by Root Canal Sealer Composition: An in Vivo and in Vitro Investigation. Pesquisa Brasileira em Odontopediatria e Clínica Integrada, v. 22, . (09/16882-7, 08/02776-8)
GARCIA PAULA-SILVA, FRANCISCO WANDERLEY; MANFRIN ARNEZ, MAYA FERNANDA; CHAVES LAMARQUE, GIULIANA DE CAMPOS; PETILLE, RENATO; RIBEIRO-SANTOS, FERNANDA REGINA; DE SENA, MARINA FERNANDES; NELSON-FILHO, PAULO; BEZERRA DA SILVA, LEA ASSED. Osteoclast formation, inflammation, and matrix metalloproteinase-9 are downregulated in bone repair following root canal treatment in dogs teeth. CLINICAL ORAL INVESTIGATIONS, v. 25, n. 7, p. 4699-4707, . (09/16882-7, 19/02060-7)

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