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SYNERGISICAL ANTIBACTERIAL AND ANTIBIOFILM ACTION OF EXTRACTS OF JABUTICABA (Myrciaria cauliflora) AND CARQUEJA (Baccharis trimera) ON MULTI-RESISTANT STRAINS OF Pseudomonas aeruginosa AND Acinetobacter baumannii

Grant number: 24/04658-5
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): July 01, 2024
Effective date (End): December 31, 2024
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Luciane Dias de Oliveira
Grantee:Sthéfani de Oliveira Dias
Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

The pathogens Acinetobacter baumannii and Pseudomonas aeruginosa have become a major public health problem as they resist several classes of antibiotics and their ability to form biofilms further increases their resistance. Therefore, it is extremely important to evaluate the antibacterial action of the synergism between herbal medicines as a way of increasing their action against infections caused by these bacteria. In this study, the antibacterial and antibiofilm action of the synergism of carqueja extract (Baccharis trimera) and jabuticaba (Myrciaria cauliflora) will be evaluated on three multidrug-resistant clinical strains and a standard strain of A. baumannii and P. aeruginosa, in addition, activity analysis will be carried out. antioxidant and phytochemistry (flavonoids, phenols and liquid chromatography-HPLC) to identify the components of the extract. For this, the jabuticaba and carqueja extract will be prepared and its soluble solids content will be quantified. In the analysis by high performance liquid chromatography (HPLC), an analytical method used in the HPLC equipment will be used, and the determination of the antioxidant action by the 2,2'-diphenyl-1-picrylhydrazyl radical reduction assay (DPPH). The determination of the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) will be in accordance with the microdilution test in broth recommended by CLSI M7- A9. Subsequently, the antibiofilm action of the extract will be analyzed by the MTT test, using predetermined minimum bactericidal concentrations (MBC), in contact with the biofilm in formation for possible inhibition, and for 24 hours in formed biofilms. The data will be analyzed by Graphpad prism 5.0 software with the ANOVA test complemented by the Tukey test with 5% significance (p<0.05).

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