Physical-chemical and antimicrobial properties of water activated by a dielectric barrier discharge (DBD) plasma produced under different levels of humidity

Abstract

Traditional irrigation methods in Endodontics may have limitations as it is a complex and clinically challenging process, as it is difficult to completely remove the biofilm, not reaching certain regions of the canal system due to anatomical issues. Studies report that conventional endodontic irrigants, despite demonstrating antimicrobial action, have toxic potential to the involved and surrounding tissues. In this context, plasma-activated water appears, which demonstrates effective antimicrobial activity, associated with anti-inflammatory and tissue repair-inducing effects. Low toxicity is an advantage reported in the literature. The present project aim investigates the physical-chemical and antimicrobial properties of water activated by a Dielectric Barrier Discharge (DBD) plasma under different levels of humidity. The research involves activating water using a DBD plasma device while varying the humidity levels during the process. Comprehensive analyses will be conducted to study the process of activation through techniques like high speed imaging, the distribution of species by KI-starch assay and to evaluate the impact of plasma activation on water's pH, oxidation-reduction potential, conductivity, total solid dissolves, and species concentration in these conditions. Furthermore, the antimicrobial efficacy of plasma-activated water against E. faecalis and C. albicans will be examined. The findings are expected to provide insights into the optimal conditions for producing plasma-activated water with potent antimicrobial properties for improving endodontic irrigation by offering a novel and effective approach to combating persistent infections and enhancing overall treatment outcomes.