Advanced search
Start date
Betweenand

Molecular and phenotypic characterization of Staphylococcus spp. methicillin-resistant strains isolated from dogs, humans, and house dust

Grant number: 23/11688-5
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): April 01, 2024
Effective date (End): July 31, 2025
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Ilana Lopes Baratella da Cunha Camargo
Grantee:Luciana Roberta Torini de Souza
Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil

Abstract

Staphylococcus are gram-positive bacteria found on humans' and animals' skin and mucous membranes and are widely distributed in soil, water, and food. The Staphylococcus genus includes more than 80 species and subspecies, with Staphylococcus aureus and Staphylococcus epidermidis being the most well-known. Despite being part of the normal human microbiota, these microorganisms can cause diseases ranging from simple infections to severe and fatal infections. From a genetic standpoint, Staphylococcus spp. have a genome of around 3 megabases (Mb), containing genes encoding virulence factors and genes that confer antibiotic resistance. As they are present on the skin and mucous surfaces of humans and animals, staphylococci are in contact with a wide diversity of bacteria, leading to a high degree of genomic plasticity. Thus, genetic materials can be exchanged among Staphylococcus in an intra-species and inter-species exchange and inter-genus exchange, meaning between Staphylococcus and other gram-positive bacteria. This exchange confers different degrees of virulence and primarily resistance to antimicrobials. This study will investigate the diversity of methicillin-resistant Staphylococcus species and characterize isolates from human communities, dogs, and household dust regarding resistance and virulence. All methicillin-resistant isolates will be fully characterized phenotypically and genotypically, as their genomes will be sequenced using both Illumina and Nanopore technologies, resulting in a complete genome. This will also enable the study of plasmids and other mobile genetic elements in this bacterial genus to understand if there is evidence of genetic exchanges between species. Automating the Phoenix M50 equipment will determine identification and sensitivity profiles. Additionally, sensitivity to antimicrobials will be determined through disk diffusion and/or determination of minimum inhibitory concentration through broth microdilution. This way, it will be possible to identify which species are most prevalent in the community of São Carlos (SP), which show the highest antimicrobial resistance, and investigate the mechanisms leading to this phenotype.

News published in Agência FAPESP Newsletter about the scholarship:
More itemsLess items
Articles published in other media outlets ( ):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Please report errors in scientific publications list using this form.