ANALYSIS OF ANTIFUNGAL ACTIVITY AND BIOSAFETY OF ANTISEPTICS USING IN VITRO AND EX VIVO MODELS

Abstract

Candida parapsilosis is a fungal pathogen that poses a significant threat to global health due to its ability to induce serious infections, often resulting in high mortality rates in several countries. Its ability to persist on surfaces can lead to outbreaks in healthcare facilities. Clearly, infection prevention strategies, including rigorous hand disinfection, are crucial to controlling Candida transmission. Unfortunately, there is a lack of data on the effectiveness of antiseptics commonly used in healthcare settings against this pathogen. The main objective of the present study will be to investigate the in vitro susceptibility of Candida parapsilosis strains obtained from the healthcare workers' hands when exposed to widely used antiseptics, both against planktonic cells and against biofilm formations. To evaluate antifungal activity, in vitro tests will be used, such as the disc-based quantitative carrier test and the dry biofilm test. Furthermore, an ex vivo pig skin model will be used to evaluate the safety of these antiseptics. This study will provide valuable insights into ongoing efforts to prevent infections, emphasizing the need to maintain a comprehensive approach to hygiene protocols in healthcare settings.