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Systemic ozone therapy associated with the use of local therapies in the prevention of drug-induced osteonecrosis of the jaws in ovariectomized rats

Grant number: 23/03757-7
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): February 01, 2024
Effective date (End): February 28, 2025
Field of knowledge:Health Sciences - Dentistry - Oral and Maxillofacial Surgery
Principal Investigator:Ana Paula Farnezi Bassi
Grantee:Izabela Fornazari Delamura
Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil


Drug-induced osteonecrosis of the jaw (MRONJ) is a condition that requires investigations to establish consensus on prevention and treatment. Given this, secondary therapies can play an important role in tissue control and response. Research has shown that systemic ozone therapy has positive results in reparative processes, and its association with topical antimicrobial agents such as Metronidazole-based Paste and 2% chlorhexidine gel may be promising in the search for adjuvant therapies that can improve the repair process in MRONJ. The objective of this work will be to evaluate the topical performance of a paste based on metronidazole and 2% chlorhexidine gel, associated with the systemic use of ozone therapy in the prevention of MRONJ after the extraction procedure in ovariectomized senile rats and submitted to therapy with Zoledronate (100¼g /kg). For this, 42 12-month-old female rats will be divided into 7 groups (n=6). In the SAL group the rats will be submitted to applications of 0.9% sodium chloride solution and in the ZOL group will receive application of Zoledronate 100¼g/kg, both every 3 days for 7 weeks; the ZOL group: ZOL+OZ; ZOL+CLX; ZOL+MTZ; ZOL+OZ/MTZ; ZOL+OZ/CLX, will receive application of zoledronate following the same protocol and topical application of paste or gel in the region of the lower 1st molar, associated or not with intraperitoneal administration of Ozone (0.7mg/kg), right after extraction of the 1st lower left molar, on the second and fourth day after extraction, and the extraction of the molar will be performed in the third week of the experiment. Euthanasia will be performed in the seventh week of the experiment, 28 days after tooth extraction. The mandibles will be resected, reduced and prepared for micro-CT, histological/histometric, immunohistochemical and inflammatory profile analyses. The data will be submitted to the normality curve to determine the most appropriate test (p<0.05).

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