In vitro evaluation of the modulation of purinergic receptors P2Y6, P2Y12, and P2X7 in a microglial cell line

Abstract

Parkinson's Disease is a neurological disorder characterized by motor and cognitive impairments, such as tremors, postural instability, muscular rigidity, and bradykinesia. Its repercussions can significantly impact behavior and homeostasis, and its pathophysiology is not yet fully understood. As a neurodegenerative disease, it is known that inflammation plays a significant role in the progression of the condition, with microglial cells being the key players in the inflammatory process. Therefore, this project aims to study the expression and modulation of purinergic receptors, specifically P2Y6, P2Y12, and P2X7, on the microglial membrane when pathologically activated by damage-associated molecular patterns (DAMPs) released by affected neurons in the nigrostriatal dopaminergic pathway. The study of these receptor activities contributes to understanding the pathogenesis of Parkinson's disease, as well as to the development of new therapeutic strategies.