Association of antibacterial and remineralizing agents in resin infiltrant composition for the treatment of initial caries lesions

Abstract

This study aims to evaluate the effects of incorporating the antibacterial monomer DMAHDM (2.5% or 5%) with or without NACP (2%) on the antibacterial potential and remineralizing potential of an experimental resinous infiltrant. Real-time metabolic status of non-disrupted Streptococcus mutans biofilms (UA 159 Luc-ldh) will be assessed using a high-throuput bioluminescence assay (n=18/group), the 3-dimensional distribution of biofilm components (nucleic acids, proteins and EPS) using a concurrent staining protocol and confocal microscopy analysis (n=3 specimens/group; 3 locations/specimen), and the quantification of the total biovolume (¼m3), total number and percentage of live cells of the bacterial biofilm of Streptococcus mutans through the Live/Dead assay (n=12 specimens/group, 3 locations/specimen). Bioactivity analysis will also be performed through Scanning Electron Microscope and X-ray diffraction analysis (n=12). Data will be analyzed for normality and homoscedasticity, and statistical analysis will be performed with appropriate tests with a significance level of 5%. (AU)