EXTRACTION AND HYDROLYSIS IN AN AUTOMATED IN-LINE REACTOR AS A TECHNOLOGY FOR THE VALUATION OF CAMBUCI PEEL (Campomanesia phaea Berg.)

Abstract

The extraction and hydrolysis technology in subcritical water is green and fast, capable of extracting and hydrolyzing higher value-added compounds from food industry waste. The objective of this work is to evaluate the effect of extraction and hydrolysis with subcritical water in obtaining sugars and bioactive compounds from cambuci peel through an automated system (PHWE/SubCWH × SPE × HPLC-PDA/RI), which is based on the coupling of pressurized hot water extraction (PHWE) and sequential subcritical water hydrolysis (SubCWH) with in-line purification by solid phase extraction (SPE) and in-line analysis by high-performance liquid chromatography (HPLC) using an array of photodiodes (PDA) and refractive index (RI) detectors. The cambuci bark extraction and hydrolysis process will be done using a central composite design (CCDR) with three independent variables, including six axial points and three replications at the central point, totaling 17 experiments. Tests will be carried out in the automated in-line system under different extraction and hydrolysis conditions: 40-120 ºC temperature; 15 Mpa pressure; 4 to 7 pH and 5 to 13 mL.min-1 flow rate to obtain bioactive compounds and sugars from Cambuci bark. Finally, a technical-economic analysis of the hydrolysis in subcritical water of the Cambuci bark will be made using methods such as Net Present Value (NPV), Internal Rate of Return (IRR), and Time of Return on Capital (TRC-Payback).