Grant number: | 23/01916-0 |
Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
Effective date (Start): | June 01, 2023 |
Effective date (End): | May 31, 2024 |
Field of knowledge: | Biological Sciences - Immunology - Immunogenetics |
Principal Investigator: | Camila Ferreira Bannwart Castro |
Grantee: | Luise Alves Tini |
Host Institution: | Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil |
Abstract MICA and MICB molecules are constitutively expressed in fibroblasts, monocytes and epithelialcells, however, their expression is stimulated in conditions such as cancer, infections andautoimmune diseases, acting as a marker of cellular stress. The MICA and MICB genes areencoded on chromosome 6 in the Major Histocompatibility Complex (MHC) region, the mostvariable region of the human genome. Variants of these genes seem to be associated with adifferential profile of expression of molecules on the surface of cells and the release of soluble2molecules, modulating the immune system by interacting with the NKG2D receptor, present innatural killer cells and subpopulations of T lymphocytes. to evaluate the molecular functionalityof MICA and MICB in healthy individuals, after identifying different haplotypes that can modifythe transcriptional profile of genes, their editing patterns and production of soluble molecules, inmononuclear cells, in physiological situations and after stimulation. For this, we will selectsamples with a characteristic genetic profile after sequencing by NGS (e.g., carriers of nonsynonymousvariants), to perform mononuclear cell culture. Then, we will quantify the expressionof MICA and MICB through surface proteins, before and after stimulation with hydrogen peroxideor LPS. The data will be correlated with the genetic profile of the samples (obtained in previoussteps). Demonstrating the heterogeneity of expression of these molecules on the cell surfaceaccording to the haplotype found can serve as a basis for understanding the dynamics betweenMICA and MICB molecules in different pathological situations or transplants, in addition tohelping in studies of associations with diseases. | |
News published in Agência FAPESP Newsletter about the scholarship: | |
More itemsLess items | |
TITULO | |
Articles published in other media outlets ( ): | |
More itemsLess items | |
VEICULO: TITULO (DATA) | |
VEICULO: TITULO (DATA) | |