Potential regulation of novel lncRNA and circRNA in lipid metabolism of muscle and liver of pigs fed different oil sources

Abstract

Lipids act as signaling molecules, participate in several processes, and are important for all cell types. The liver, adipose tissue, and skeletal muscle are the main tissues responsible for lipid metabolism, a complex and dynamic process involving many transcriptional regulators. The lncRNA and circRNA are key regulators in metabolism, play a key role with respect to lipids, and have been widely studied in animal models such as pigs. For this project, data from 72 male pigs from a study with a 98-day experimental period will be used. The experimental treatments consisted of corn-soybean meal diets containing 1.5% soybean oil (SOY1.5), or 3.0% soybean oil (SOY3.0), or 3.0% canola oil (CO), or 3.0% fish oil (FO). Liver and muscle samples were collected and total mRNA extraction was performed by Illumina technology. With the sequencing data, new transcripts and lncRNA and circRNA will be identified. With the new lncRNA and circRNA, differential expression analysis will be performed by DESeq2 statistical package and functional enrichment analysis will be done by PANTHER and DAVID software. Thus, the hypothesis of this work is that diets with different sources of oil fed to pigs in the phases of growth and termination, may affect the gene expression profile of novel lncRNA and circRNA and affect processes associated with muscle and liver lipid metabolism. As a main goal we will identify novel lncRNA and circRNA in muscle and liver of pigs fed different sources of oil and verify how the diets modulate the gene expression in different tissues.