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EVALUATION OF ROUGHNESS AND MICROHARDNESS OF ERODED ENAMEL TREATED WITH RESINOUS INFILTRANT CONTAINING AMORPHOUS CALCIUM PHOSPHATE AND SUBMITTED TO ABRASION.

Grant number: 22/16194-8
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): May 01, 2023
Effective date (End): April 30, 2024
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal Investigator:Giselle Maria Marchi
Grantee:Ingrid de Oliveira Priori
Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

This study proposes to evaluate the surface roughness and microhardness of eroded enamel treated with an experimental resinous infiltrator containing 2% amorphous calcium phosphate and subjected to simulated brushing. For this study, enamel/dentin samples will be made from healthy human third molars, which will be randomly assigned to three different treatment groups (n=20): G1 - Commercial Infiltrant, G2 - Experimental Infiltrant and G3 - Artificial Saliva and, stored in eppendorfs under relative humidity. Prior to the treatments, the samples will undergo a simulation of intrinsic erosion, where they will be immersed in demineralizing solutions (DES), composed of 0.01 M HCl (pH 2.3), for 2 min, and remineralizing solutions (RE), composed of 150 mM KCl, 1.5 mM CaCl2, 0.9 mM KH2PO4, 0.1 M Tris (pH 7.0), for 60 min. Four cycles per day will be performed for 5 days. After that, the samples will be submitted to resin infiltration and exposure to artificial saliva, according to each group. The analyses of surface roughness and microhardness will be performed in 4 different times: time I - healthy enamel, time II - eroded enamel, time III - post-treated enamel, and time IV - treated enamel after simulated brushing. For the simulated brushing, a mass of 8g of a dentifrice mixed with 24ml of distilled water will be used for each sample, forming a slurry (1:3). It will be performed 10.000 mechanical brushing cycles, with a frequency of 4 Hz, at 37o± 0.5oC, under a load of 200 g to simulate the force used during brushing. The results will be submitted to homogeneity and normality tests to be further analyzed by the most appropriate test, considering a 5% significance level.

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