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Interrogating the nuclear genome organization in Trypnanosoma cruzi replicative and non-replicative life forms by using high-throughput chromosome conformation capture (Hi-C) approach

Grant number: 22/14056-7
Support Opportunities:Scholarships abroad - Research Internship - Post-doctor
Effective date (Start): February 13, 2023
Effective date (End): April 12, 2023
Field of knowledge:Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
Principal Investigator:Julia Pinheiro Chagas da Cunha
Grantee:Natália Karla Bellini
Supervisor: Nicolai Siegel
Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
Research place: Ludwig Maximilian University of Munich (LMU Munich), Germany  
Associated to the scholarship:21/03219-0 - Interrogation of a potential transcription regulation in Trypanosoma cruzi by evaluation of nascent transcripts genome-widely, BP.PD


This proposal is designed to address nuclear three-dimensional features of two life forms of the parasite Trypanosoma cruzi, and to investigate its probable impacts on different aspects of T. cruzi biology. Gene expression in trypanosomes is broadly determined by post-transcriptional mechanisms, however, a significant decrease in global transcription has been observed if comparing their life forms, suggesting some layer of transcription regulation. Additionally, T. cruzi are subjected to many epigenetic changes (e.g., histone PTMs, nucleosome differential occupancy, different levels of open chromatin, etc) that changes along parasite life and cell cycle. The 3D genome organization was shown to be important on monogenic expression of antigenic variant surface glycoproteins in T. brucei. We hypothesize that the 3D genome organization in the T. cruzi can also be one additional layer of gene expression regulation. Thus, we propose to standardize and obtain high-throughput chromosome conformation capture (Hi-C) assays of T. cruzi to characterize their 3D landscape of chromatin in two life forms. For this proposal, bioinformatic pipelines will be applied and data will be explored preliminary to further elucidate possible interplays between the nuclear architecture and epigenomic profile in T.cruzi. (AU)

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