Different Biomolecules used in Functionalized Implants and their performance in the perimplantar repair of estrogen deficient rats. Biomechanical and molecular analysis

Abstract

Post-menopausal hormonal changes favor the onset of bone diseases such as osteoporosis, which can negatively affect bone turnover. A strategy that has been widely used with the objective of improving the bone repair process is the surface functionalization of implants, with the objective of locally adding biomolecules that can favor the repair steps. In this sense, two substances have relevant properties for the repair steps, namely, genistein and teriparatide. The objective of this work is to characterize, from biomechanical analysis (counter-torque) and real-time PCR, the osseointegration and peri-implant repair provided by the addition of genistein and teriparatide in different concentrations to the surface of implants in the tibia in normal and female rats. under conditions that mimic osteoporosis. For this 48 female rats, N=48, (Rattus norgicus albinus, Wistar) will be used, approximately 300g, divided into 2 groups: SHAM (animals submitted to sham bilateral ovariectomy surgery) and OVX (animals submitted to bilateral ovariectomy surgery), each group will have 24 rats, divided into 3 subgroups according to functionalization, with 8 animals each (Conv, Gen 100mM, Teri 10ng). After 30 days of the fictitious and real ovariectomy, all animals will undergo implant placement surgery in both tibial metaphyses. Ninety-six commercially pure grade IV titanium implants will be used with conventional or teriparatide and genistein-treated surfaces. The animals will be euthanized 28 days after implant placement and the tibias will be destined for the following analyses: Real-time PCR (IBSP, ALP, OCN, VEGF) on the right tibia, while counter-torque will be performed on the left for evaluation biomechanics. The quantitative data will be submitted to the normality test and this will indicate the most appropriate statistical test. For all tests, a significance level of 5% will be adopted.