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Synthesis, characterization and efficacy of an experimental bleaching agent based on hyaluronic acid and containing titanium dioxide nanoparticles co-doped with F and N: in vitro study and clinical evaluation.

Grant number: 21/14834-7
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): December 01, 2022
Effective date (End): February 28, 2025
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal Investigator:Vanessa Cavalli Gobbo
Grantee:Priscila Borges Gobbo de Melo
Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil


The development of a safer and more effective whitening gel, with bioactive properties and less likely to develop tooth sensitivity, is necessary. Thus, this study aims to synthesize and characterize the efficacy and safety of an experimental bleaching agent based on hyaluronic acid (HA) and containing NF_TiO2 nanoparticles, in vitro and in vivo. The project will be divided into 2 Studies. In Study 1, enamel specimens will be treated with: hydrogen peroxide (PH, 6 and 35%, commercial control), carbomer 940 (CAR) or AH + PH 0, 6 and 35% + NF_TiO2 (0, 5 and 10 %) with and without light and negative control (no treatment). The bleaching will be carried out in 3 sessions of 40 min with an interval of 7d. The following will be evaluated: Phase 1: characterization of experimental gels [pH, decomposition, X-ray diffraction (XRD) and transmission electron microscopy (TEM)]; Phase 2: color change [spectrophotometer (L*, a*, b* parameters), lightening index (WID) and color change (”E)]; Phase 3: mineral content [roughness, surface microhardness (KHN), energy-dispersive X-ray spectroscopy (EDS) and scanning electron microscopy (SEM)]; Phase 4: cytotoxicity (MTT) and cell morphology (SEM). Study 2 will be a blind randomized clinical trial. The experimental design follows the rules established by CONSORT 2010 and patients will be selected according to the eligibility criteria (inclusion and exclusion criteria). Afterward, they will be submitted to treatments (PH 35% commercial and gel-based on CAR and AH). Assessments of color change, tooth sensitivity testing (VAS), and oxygen saturation (SaO2) levels will be performed. In both studies, data will be submitted to statistical analysis and, according to normality, adequate tests will be performed (± = 5%).

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