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Acquired pellicle engineering for the control of dental erosion: in vitro evaluation of the protective potential of different concentrations of sodium trimetaphosphate associated or not with CaneCPI-5

Grant number: 22/11297-3
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): December 01, 2022
Effective date (End): November 30, 2024
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Marília Afonso Rabelo Buzalaf
Grantee:Karolyne Sayuri de Araujo Kitamoto
Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
Associated research grant:19/26070-1 - Modulation of acquired enamel pellicle to control dental mineral loss: unravelling mechanisms to make therapies possible, AP.TEM

Abstract

Sodium trimetaphosphate (TMP) has been studied for its incorporation into fluoride vehicles for topical use. It is known that it promotes a significant increase in the preventive and therapeutic potential of fluoride. The incorporation of proteins (eg, CaneCPI-5) into the acquired enamel pellicle (AEP) has favored its ability to protect against erosion. In this context, associating CaneCPI-5 with TMP can enhance its protective effect, aiming at its incorporation into dental products to increase the ability of the AEP to protect against acids. Thus, the aim of the present study is to analyze the protective effect and evaluate different TMP concentrations associated or not with CaneCPI-5 toprotect against initial enamel erosion in vitro. 153 samples (4 X 4 mm) of bovine enamel will be prepared. Subsequently, the samples will be randomized and divided into 9 groups, according to the treatments (n/group=17): Deionized water (negative control); Commercial solution with SnCl2/NaF/AmF (800 ppm Sn+2,500 ppm F-, pH 4.5, Erosion Protection® - GABA, (positive control 1); 0.1 mg/mL CaneCPI-5 (positive control 2); 0.5%; microparticulated TMP 1.0% microparticulated TMP ; 3.0% microparticulated TMP ; 0.5% microparticulated TMP + 0.1 mg/mL CaneCPI-5; 1.0% microparticulated TMP + 0.1 mg/mL CaneCPI-5; 3.0% microparticulated TMP + 0.1 mg/mL CaneCPI-5. Initially, the samples will be treated individually (250 µL) with the respective solutions for 2h, 30ºC, under constant agitation. PAE will be formed individually (250 µL) for 2 h, 30ºC, under constant agitation (stimulated saliva will be collected from 3 volunteers). These procedures will be performed once a day, for 3 consecutive days. Surface microhardness (SHM) analyses will be performed and the changes in SMH (SHM baseline - Post-erosion SMH) will be calculated. Data will be analyzed using the GraphPad Prism software. First, the data will be checked for normality (Kolmogorov-Smirnov test) and homogeneity (Bartlett test) to select the appropriate statistical test. The significance level will be 5%.

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