Evaluation of 10% metronidazole and 2% lidocaine paste with ozone therapy for the treatment of Zoledronate-induced osteonecrosis of the jaws after tooth extraction in ovariectomized rats

Abstract

Drug-induced osteonecrosis of the jaw (MRONJ) is a pathological condition that still does not present a consensus in the literature on its treatment and therefore deserves attention. In view of this, secondary therapies may play an important role in tissue control and response, while metronidazole-based pastes for topical use may be promising. The objective of this study will be to evaluate the topical performance of metronidazole-based pastes associated with ozone therapy in the treatment of drug-induced osteonecrosis of the jaws after toothextraction in ovariectomized senile rats submitted to zoledronate therapy. For this, 15 rats of 12 months of age, will be divided into 3 groups. In the SAL group the rats will be submitted to applications of 0.9% sodium chloride solution and the ZOL group will receive application of Zoledronate 100¼g/kg, both every 3 days for 7 weeks; the ZOl+OZ/POI group will receive application of zoledronate following the same protocol and topical application of the pasteintended for each group, in the alveolus after the extraction of the 1st lower left molar, on the day of extraction, 2 and 4 days later, in addition to ozone therapy. Euthanasia will be performed in the seventh week of the experiment, 28 days after tooth extraction. In addition, the characterization of bone architecture in senility will be performed on day 0 (MCT0), after 3 weeks of application of zoledronate (MCT1), comparing with the group that will not receive zoledronate (MCT2), totaling 15 animals (n=5) . The mandibles will be resected, reduced and prepared for micro-CT, histological/histometric analysis. The data will be submitted to the normality curve to determine the most appropriate test (p<0.05).