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Experimental model for evaluation of therapy photodynamics associated with chemical sclerotherapy for the treatment of veins in rabbits ears

Grant number: 22/06684-8
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): August 01, 2022
Effective date (End): July 31, 2023
Field of knowledge:Health Sciences - Medicine - Surgery
Principal Investigator:Matheus Bertanha
Grantee:Marcos Vinicius de Souza
Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil


Lower limb varicose veins have a general prevalence estimated at 57.5% of women and are considered mild when they do not present pain, generally limited to telangiectasias and reticular veins. There are some possible techniques for treatment, among the most common are chemical sclerotherapy, cryosclerotherapy, laser photothermolysis, electrocoagulation and radiofrequency. The possible use of photodynamic therapy with the same objective and better results is highlighted. Objective: To evaluate the safety and efficacy of the photodynamic method associated with conventional sclerosing agents versus conventional sclerotherapy for the treatment of vessels in an experimental rabbit ear model. Methods: Experimental study where 30 female rabbits will be used. The animals will be sedated to perform all procedures. The treatment will be carried out in the dorsal vein of the ear. The animals will be divided into 6 groups: G1: Control; G2: Sclerotherapy with 70% glucose and 0.1% polidocanol; G3: Photodynamic therapy (808nm laser + 5mg/mL ICG) associated with sclerotherapy with 70% glucose and 0.1% polidocanol; G4: Sclerotherapy with 70% glucose and 0.1% polidocanol in association with 808nm laser; G5: Sclerotherapy with 70% glucose and 0.1% polidocanol with an association of 5mg/mL ICG; G6: 5mg/mL ICG injection and 808nm laser application. The area will be photographed and quantitatively evaluated by comparing the objective measurements obtained on days (D) D0, D1, D3, D5, D7, and D10. Quantification will be done using the ImageJ" software segmental tool. Thermographic analysis of the ears will also be performed, and after the euthanasia of the animals, histological evaluation of the ear fragment will be performed by HE and special histology by Verhoeff stain. The data will be statistically compared to obtain the results.(AU)

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