Scholarship 21/13317-9 - Consumo de álcool por menores, Transtornos induzidos por álcool - BV FAPESP
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Acute restraint stress during adulthood modulates glutamate release through CRF type 1 receptors in bed nucleus of stria terminalis of male and female mice with history of adolescent alcohol exposure

Grant number: 21/13317-9
Support Opportunities:Scholarships abroad - Research Internship - Doctorate
Start date until: August 15, 2022
End date until: August 14, 2023
Field of knowledge:Biological Sciences - Pharmacology - Neuropsychopharmacology
Principal Investigator:Fabio Cardoso Cruz
Grantee:Natalia Bonetti Bertagna
Supervisor: Tiffany Wills
Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
Institution abroad: Louisiana State University Health Sciences Center New Orleans, United States  
Associated to the scholarship:20/04389-3 - Maternal separation stress and behaviors related to alcohol dependence in mice: assessment of CRFergic neurotransmission, BP.DR

Abstract

Adolescence is an important period for neuroadaptations that contributes to the normal maturation of central nervous system. Alcohol consumption usually initiates in adolescence, and it is one of the strongest predictors for the development of an alcohol use disorders (AUDs). N-methyl-D-aspartate (NMDA) receptors (NMDARs) are present along over the stages of development and they are implicated in the pathophysiology of AUDs. The Bed Nucleus of Stria Terminalis (BNST) is a critical regulator of stress and reward responses. However, it is unclear whether the alcohol-induced modifications in adolescent BNST are preserved in adulthood and if they are different between male and female mice. The aim of this project is to assess if chronic intermittent alcohol vapor (AIE) during the adolescence produces changes in the glutamatergic transmission into the female and male mice BNST. Further, whether acute stress during adulthood is able to increase glutamate release by presynaptic CRF 1 receptors (CRFR1). In exp 1, on post-natal day (PND 21) male and female C576J/black mice will be exposed to AIE or air. In adulthood (PD70) they will undergo to restraint stress for 1-hour. After, BNST slices will be collected for electrophysiology. In exp 2, adolescent mice (PND 21) will be exposed to AIE or Air. On PND 70, they will be intraperitonially injected with CRFR1 antagonist or vehicle 30 min prior to restraint stress. After, BNST slices will be collected for electrophysiology, in which, the effect of the Ro 25-6981 (a NMDA blocker) or DHPG (group 1 mGluR agonist) will be evaluated. For behavioral analysis mice will be submitted to AIE, and on PND 70, CRFR1 antagonist or vehicle will be administered 30 min prior to the restraint stress and then mice will be submitted to the novelty-induced hypophagia task (NIH) and elevated plus maze (EPM). (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BERTAGNA, N. B.; WILSON, L.; HOLMGREN, E. B.; CRUZ, F. C.; ALBRECHET-SOUZA, L.; WILLS, T. A.. Hyperkatifeia and CRF signaling in the bed nucleus of STRIA terminalis (BNST) after adolescent alcohol exposure in female mice. ALCOHOL-CLINICAL AND EXPERIMENTAL RESEARCH, v. 47, p. 2-pg., . (21/13317-9)

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