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Development of a nanoemulsion containing quercetin and evaluation of its antioxidant activity

Grant number: 22/00709-9
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): May 01, 2022
Effective date (End): April 30, 2023
Field of knowledge:Health Sciences - Pharmacy - Pharmaceutical Technology
Principal researcher:Priscyla Daniely Marcato Gaspari
Grantee:Júlia de Cássia Pavezi
Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


The skin is always exposed to oxidizing and inflammatory agents, which can denature proteins, alter apoptosis and influence the release of pro-inflammatory mediators. Therefore, it is necessary to strengthen the skin's defense mechanism with antioxidants. Considering this, Quercetin is a flavonoid with antioxidant properties, acting as a reducing agent in addition to inhibiting oxidative stress. However, Quercetin presents low bioavailability, skin permeation and stability. To overcome these limitations, this flavonoid can be encapsulated in nanostructured systems. Thus, the aim of the project is to prepare and characterize an olive oil nanoemulsion containing quercetin and to evaluate its cytotoxicity and antioxidant activity in skin cells. For this, nanoemulsions with and without quercetin will be prepared by the emulsion and agitation method in ultra-turrax. These nanoemulsions will be characterized in terms of diameter, polydispersity index, and zeta potential by the dynamic light scattering (DLS) technique. The pH and electrical conductivity will be measured by a pH meter and a conductivity meter, respectively. The stability of the nanoemulsions, storage at 25oC, will be evaluated by DLS. In addition, the encapsulation efficiency will be evaluated by UPLC-MS. The formulation's cytotoxicity will be evaluated in BALB/3T3 fibroblasts and HaCaT keratinocytes. Finally, the antioxidant activity of the nanoemulsion will be evaluated by the DPPH method, and in 3T3 cells using the fluorescent intracellular probe DCFH-DA. It is expected that the formulation has a high capacity to incorporate quercetin with low toxicity and high antioxidant activity.(AU)

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