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Development and application of bioinformatic pipelines for metagenomic analysis of clinical samples

Grant number: 22/00910-6
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): March 01, 2022
Effective date (End): December 31, 2022
Field of knowledge:Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
Principal Investigator:Svetoslav Nanev Slavov
Grantee:Gabriel Montenegro de Campos
Host Institution: Hemocentro de Ribeirão Preto. Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto da USP (HCMRP). Secretaria da Saúde (São Paulo - Estado). Ribeirão Preto , SP, Brazil
Associated research grant:17/23205-8 - Evaluation of the impact of emerging and reemerging viruses in the field of hemotherapy and stem cell transplantation by multiple-research approach, AP.JP

Abstract

The metagenomic approaches are one of the most sensitive tools to identify emerging viruses. With the emergence of the New Generation Sequencing (NGS) technologies, the software diversity used for data analysis has grown significantly. The bioinformatic analysis of the obtained NGS sequencing data represents still a significant challenge for the correct interpretation of the data due to the variability of the programs used for taxonomic classification. Therefore, this project aims to develop and optimize a pipeline for viral metagenomic analysis originating from NGS sequencing data obtained from several study groups (plasma samples obtained from patients with prostate cancer, undefined febrile illness, and waste pickers). At the same time, parallel comparisons with other bioinformatics programs and mainly viral taxonomy classifiers will be carried out. Adjustments will be performed to the classifiers' databases once they represent high variability of the available viral taxonomic data. Once this is performed, the identified viruses with clinical importance will be assembled and they will be subjected to phylogenetic analysis in order to investigate their molecular epidemiology. This proposal will allow a deeper insight into the metavirome of different groups of patients.(AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ZANETTE, DALILA LUCIOLA; COELHO, KAROLINE BRITO CAETANO ANDRADE; DE CARVALHO, ENEAS; AOKI, MATEUS NOBREGA; NARDIN, JEANINE MARIE; LALLI, LARISSA ARAUJO; BEZERRA, RAFAEL DOS SANTOS; GIOVANETTI, MARTA; ZUCHERATO, VICTORIA SIMIONATTO; DE CAMPOS, GABRIEL MONTENEGRO; et al. Metagenomic insights into the plasma virome of Brazilian patients with prostate cancer. MOLECULAR & CELLULAR ONCOLOGY, v. 10, n. 1, p. 9-pg., . (19/08528-0, 22/00910-6, 17/23205-8, 20/13194-1)

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