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Expression of the pSAMBA-GUS-GFP reporter gene at different stages of development of the Micro-tom tomato

Grant number: 21/13844-9
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): December 01, 2021
Effective date (End): November 30, 2022
Field of knowledge:Biological Sciences - Biochemistry - Molecular Biology
Cooperation agreement: Max Planck Society for the Advancement of Science
Principal researcher:Nubia Barbosa Eloy
Grantee:Gabriela de Fátima Cia
Home Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
Associated research grant:17/10333-8 - APC as an entry point to study small molecule regulation of the cell cycle, AP.JP

Abstract

The Anaphase-Promoting Complex/Cyclosome (APC/C) is an E3 ubiquitin ligase involved in ubiquitin-dependent proteolysis of key cell cycle proteins by the 26S proteasome. The spatio-temporal degradation of mitotic cyclins and securins ensures the correct onset of the cell cycle phases and exit from the cell division program, respectively. The identification of genes encoding APC/C subunits in Arabidopsis thaliana suggests that the complex has other specific functions during plant development, such as embryogenesis, gametogenesis, growth regulation, hormone signaling and symbiotic interactions. Despite extensive research on APC/C, only a few components have been characterized in plants. One of these proteins, SAMBA, proved to be a highly promising candidate in Arabidopsis with regard to increasing productivity, through production of larger leaves, roots and seeds. In this project we aim to characterize the expression of the pSAMBA-GUS-GFP reporter gene at different stages of development of the Micro-tom tomato. (AU)

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