Effect of experimental solutions and dentifrices containing polymers associated with fluoride and stannous ions in dentin permeability and tubule occlusion

Abstract

This study aims to develop experimental solutions containing fluoride and stannous ions associated film-form polymers, to promote tubular occlusion and control dentin hypersensitivity. Dentin fragments will be immersed in a 17% EDTA for 5 min and randomly distributed into 13 groups (n = 10): 1. Negative Control Group, with deionized water; 2. Group F Commercial (250 ppm F-, NaF + AmF); 3. Group F (225 ppm F-, NaF); 4. Group F + Sn (225 ppm F- and 800 ppm Sn2+, SnCl2) ; 5. MPC Group; 6. LPP Group; 7. Chitosan Group; 8. F + MPC Group; 9. F + LPP Group; 10. F + Chitosan Group; 11. F + Sn + MPC Group; 12. F Group + Sn + LPP; 13. Group F + Sn + Chitosan. The specimens will be tested on an erosive-abrasive cycling (immersion in a 0.3% citric acid solution - 5 min, followed by immersion in artificial saliva - 60min, 4x/day, for 5 days). Brushing will be performed 30 minutes after the first and the last erosive challenges. The change in dentin permeability will be determined by hydraulic conductance (% Lp). Tubular occlusion will be evaluated qualitatively and quantitatively in the micrographs. In the second phase, the polymers or associations that obtained the most promising results will be added to a dentifrice formula, which will be then tested for their ability to reduce dentin permeability and promote tubular occlusion after an erosive-abrasive cycling. The data will be statistically analyzed, considering a significance level of 5%. (AU)