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Epigenetic regulation of LDOC1 in tumor biology of MEC subpopulation in posterior fossa ependymoma Group A

Grant number: 21/11402-9
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): February 01, 2022
Effective date (End): January 31, 2023
Field of knowledge:Health Sciences - Medicine - Maternal and Child Health
Principal researcher:Luiz Gonzaga Tone
Grantee:Graziella Ribeiro de Sousa
Supervisor abroad: Nicholas Kenneth Foreman
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Research place: University of Colorado, Denver (CU), United States  
Associated to the scholarship:18/23372-4 - Study of the effects of DNA demethylation on genes associated with developmental pathways in pediatric Ependymomas, BP.DR

Abstract

Ependymoma (EPN) accounts for a substantial number of childhood deaths and over 70% of children presenting with EPN will relapse and eventually die. This tumor is a heterogeneous disease, which has been classified into nine molecular subgroups.Importantly, children with the most common type of ependymoma, posterior fossa Group A (PFA1), relapse more frequently and experience more invasive, metastatic disease at relapse. Thus, there is a critical need for more effective therapies to combat high-risk PFA1 tumors. Single-cell RNA Sequencing data suggest that the epigenetic silencing of LDOC1 within a specific subpopulation of tumor cells, mesenchymal EPN cells (MEC), has a profound, direct impact on the tumor biology of PFA1 tumors. The driving hypothesis is that epigenetic silencing of LDOC1 in the MEC subpopulation, because of chromatin remodeling, is the molecular driver in PFA1 EPN, through upregulation of NF-KB signaling. To address this hypothesis, aim one will determine the role of LDOC1 expression in EPN by examining 1) the mechanism of gene silencing, 2) the functional role of loss of LDOC1 in vitro and in vivo, and 3) the genomic transcriptional targets of LDOC1. The collective proposed studies may reveal a previously uncharacterized role of LDOC1 in MEC subpopulations of PFA1 tumors, as well as will define the effect of LDOC1 loss, which we hypothesize to be the molecular driver of tumor biology of PFA1 EPN. These studies will significantly add to our understanding of childhood EPN and have the potential to identify rational therapeutic targets for children with this high-risk, poor-outcome pediatric brain tumor.

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