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Molecular detection of the main bacterial pathogens in pleurisies of slaughter pigs

Grant number: 21/10924-1
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): December 01, 2021
Effective date (End): November 30, 2022
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Pathology
Principal researcher:Luís Guilherme de Oliveira
Grantee:Geovana Coelho Ferreira
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Porcine respiratory disease complex pigs of intensive production are one of the most responsible for economic losses within production, due to reduced daily weight gain, condemnations at slaughter, costs with control and prevention, and increased mortality. Therefore, sanitary monitoring at the swine slaughterhouse is important for epidemiological sanitary surveillance and monitoring of herd health. Among the main lesions found during the inspection of carcasses are pleurisies, which are inflammatory lesions and adherence of the pleura, a serous membrane that lines the lungs on the internal surface of the rib cage, and can be caused by various infectious agents, and cause partial or total condemnation. The aim of this study is to identify the main etiological pathogens involved in pleurisy lesions in slaughter pigs. For this purpose, a slaughterhouse located in the State of São Paulo, with a constant slaughter flow will be selected, where approximately 100 animals from 5 lots from different commercial swine farms will be selected. For these lots, the Pneumonia Index (IPP) will be determined. Among the evaluated animals that present pleurisy lesions, these will be categorized into 5 groups according to the severity of the lesion (G1- score 1; G2- score 2; G3- score 3; G4- score 4 and G5- control group, without lesions) where 10 pleural fragments will be collected for each of the groups, in association with lung fragments from the carcasses with pleurisy. Afterwards, multiplex qPCR will be performed to identify the following bacterial pathogens: Glaeserella parasuis, Streptococcus suis, Pasteurella multocida, Actinobacillus pleuropneumoniae and Mycoplasma hyopneumoniae. The normality of the variables will be verified by the Shapiro-Wilkins test. In case of normality, analysis of variance (ANOVA), simple T-test will be applied for the comparison between groups. If there is no normality, non-parametric tests (Wilcoxon) will be used. (AU)

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