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Enzymatic cascade synthesis of vitamin B6 through a xylose-based route

Grant number: 21/09659-1
Support Opportunities:Scholarships abroad - Research Internship - Doctorate (Direct)
Effective date (Start): November 01, 2021
Effective date (End): September 01, 2022
Field of knowledge:Biological Sciences - Biochemistry - Chemistry of Macromolecules
Principal Investigator:Leticia Maria Zanphorlin
Grantee:Renan Yuji Miyamoto
Supervisor: Volker Sieber
Host Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia e Inovações (Brasil). Campinas , SP, Brazil
Research place: Technical University of Munich, Straubing (TUM), Germany  
Associated to the scholarship:18/02865-2 - Molecular investigation of new xylose isomerases for application to lignocellulosic materials fermentation, BP.DD


Vitamin B6 is a fundamental nutrient involved in more bodily processes than any other vitamin. However, although vitamin B6 is essential, humans cannot synthesize it, so this micronutrient must be obtained from foods or isolated supplements. Currently, vitaminB6 may be produced by technological routes through chemical or fermentative processes. Chemical synthesis employs expensive and/or hazardous resources while biological routes still suffer to produce high titers due to toxic features of its intermediates, and its final product can interact with a wide range of metabolic reactions. Thus, we propose a bio-based production strategy via a synthetic biochemistry approach to make vitamin B6in vitro from a xylose-conversion enzyme cascade through six steps. We hope that this can be achieved through mutual knowledge exchange, where the Brazilian enzymatic background (Mr. Miyamoto and Dr. Zanphorlin) will be deeply enhanced by the German know-how on the cell-free bioprocess design (Dr. Sieber group). For this project, it was set three main objectives (I) synthesize all six genes and express in the soluble fraction;(II) Test all purified enzymes in one-pot reaction; and (III) evaluate and optimize the bioprocess through Placket-Burmann analysis and rotatable central composite design. Therefore, we expect to apply novel synthetic biochemistry techniques to develop a novel approach for vitamin B6 production to overcome the major drawbacks faced by state-of art technologies using xylose as raw-material. (AU)

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